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HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
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Diameter (Metric)

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Length (Metric)

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Particle Size

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Pore Size

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Phase

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Carbon Load

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Product Line

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Technique

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Type

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Material

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1 – 15 of 17,657 results
1

Thermo Scientific™ Hypersil™ BDS C18 Columns

Use as a robust, general-purpose column for a wide range of applications in QC/QA labs.

EDGE
Carbon Load 11%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 170 m²/g
USP Type L1
2

Thermo Scientific™ Hypersil™ ODS C18 Columns
GSA_VA

Use as a general purpose C18 phase for acidic and neutral analytes.

EDGE
Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
3

Thermo Scientific™ Hypersil GOLD™ C18 Selectivity HPLC Columns
GSA_VA

Use these endcapped, ultrapure, silica-based columns to generate exceptional peak shape and resolution for HPLC and LC/MS analyses using generic gradients

EDGE
4

Thermo Scientific™ Acclaim™ 120 C18 Columns
GSA_VA

Use these high-efficiency reversed phase C18 columns for maximum resolution in a wide variety of applications.

Pore Size 120 Å
Carbon Load 18%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 300 m²/g
USP Type L1
5

Thermo Scientific™ EASY-Spray™ HPLC Columns

Robust capillary flow HPLC columns for sensitive protein, peptide and mAbs separation using the stand alone or integrated easy to use design.

EDGE
Phase Reversed Phase
6

Thermo Scientific™ Acclaim™ Polar Advantage II HPLC Columns
GSA_VA

Resolve polar and nonpolar compounds in a single run. These columns have high pH stability (pH 1.5–10) in the separation of basic, neutral, and acidic species.

Pore Size 120 Å
Stationary Phase Polar Embedded
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
Surface Area 300 m²/g
USP Type L60
7

Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns
GSA_VA

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

EDGE
Pore Size 100 Å
Stationary Phase C18
Phase Reversed Phase
USP Type L1
8

Thermo Scientific™ BetaSil™ C18 HPLC Columns, 5μm Particle Size
GSA_VA

High surface area with high bonded phase coverage

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 20%
Stationary Phase C18
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Surface Area 310 m²/g
USP Type L1
9

MilliporeSigma™ SS Hibar™ Columns With LiChrospher™ 100 RP-18 and RP-18e Sorbents

HPLC column

Pore Size 100 Å
Particle Size 5 μm
Type Column
Stationary Phase C18
Phase Reversed Phase
Packing Material Silica
Column Type Reversed Phase
Quantity 1/Pk.
Material Stainless Steel
Surface Area 350 m²/g
USP Type L1
10

Thermo Scientific™ Acclaim™ Mixed Mode WCX-1 HPLC Columns
GSA_VA

Separate cationic molecules with powerful adjustable selectivity control using this mixed-mode reversed-phase and weak cation-exchange LC column.

Pore Size 120 Å
Stationary Phase Hydrophobic Alkyl Chain with a Carboxyl Terminus
Temperature 50°C
Phase Reversed Phase, Ion Exchange, Ion Exclusion, HILIC
Endcapped Proprietary
Column Type Mixed Mode
Green Features Responsibly packaged
Surface Area 300 m²/g
Product Line Acclaim
USP Type L85
11

Thermo Scientific™ Aquasil™ 5μm C18 HPLC Columns, 5?m Particle Size

Thermo ScientificTM AquasilTM 5μm C18 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

Pore Size 100 Å
Particle Size 5 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
12

Thermo Scientific™ Aquasil™ 3μm C18 HPLC Columns

Available in limited dimensions. Please contact Customer Service for more details.

Pore Size 100 Å
Particle Size 3 μm
Carbon Load 12%
Stationary Phase C18
Phase Reversed Phase
For Use With LC/MS
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 8
Surface Area 310 m²/g
USP Type L1
Column Format Analytical Column
13

MilliporeSigma™ Supelco™ Ascentis™ Express F5, 2.7 μm HPLC Column

The pentafluorophenylpropyl stationary phase of Ascentis Express F5 provides a stable reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines.

14

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å PFAS Delay, 2.7 μm HPLC Column

The highly retentive endcapped silane of the Ascentis™ Express PFAS Delay column provides high retention of PFAS compounds across various mobile phase conditions and is used to delay background instrument.

Pore Size 90 Å
Particle Size 2.7 μm
Temperature <60°C
Matrix Fused-Core™ Silica Particle Platform Particle Platform Superficially Porous Particle
Length (Metric) 5 cm
Column Type Reversed Phase
pH 2 to 9
Quantity 1 Ea.
Material Stainless Steel
Technique HPLC, LC/MS
Surface Area 135 m2/g
Max. Pressure 600 bar
15

Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC Columns

Separate challenging peptide mapping samples with Thermo Scientific™ EASY-Spray™ PepMap™ Neo UHPLC columns.

Pore Size 100 Å
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
For Use With (Application) Bottom-up Proteomics, LC-MS
Surface Area 450 m2/g
Max. Pressure 22,000 psi (1500 bar)
Column Format Analytical Column, EASY-Spray™
Particle Size 2 μm
Carbon Load 15%
Packing Material Spherical, Fully Porous, Ultrapure Silica
Diameter (Metric) 75 μm
pH 2 to 8
Product Line Easy Spray
USP Type L1